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VHH Discovery By Serum Proteomic Analysis

VHH Discovery By Serum Proteomic Analysis

Llama VHH discovery from serum Camelids, such as llamas and alpacas, naturally produce both conventional antibodies and heavy-chain only antibodies (HCAbs)(1). The antigen-binding domain of the HCAb, called the VHH or nanobody, is comprised of a single polypeptide...
I/L Determination in De Novo Antibody Sequencing

I/L Determination in De Novo Antibody Sequencing

I/L Determination in De Novo Antibody Sequencing Isoleucine and leucine determination De novo antibody sequencing by tandem mass spectrometry(MS/MS) uses the differences in mass of different amino acids to accurately determine the correct sequence. Distinguishing...
Constructing an Accurate Antibody Repertoire

Constructing an Accurate Antibody Repertoire

Constructing an Accurate Antibody Repertoire What is an antibody repertoire? The immune repertoire is the collection of unique immunoglobulin and T-cell receptor sequences present in an individual at a particular time. At Digital Proteomics we focus primarily on the...
From RNA to Sequencing: QC Matters

From RNA to Sequencing: QC Matters

Sequencing the immunoglobulin (Ig) repertoire provides us with important information on the adaptive immune response, and can help with the development of diagnostic and therapeutic applications. A single human can have an estimated 10¹¹ B cells with millions of...
De Novo Antibody Sequencing Process: An Overview

De Novo Antibody Sequencing Process: An Overview

De Novo Antibody Sequencing Process: An Overview If you search the internet for ‘antibody sequencing’, you will be rewarded with a long list of resources and services. The terms used to describe antibody sequencing are overloaded and highly similar. We felt a single...

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