Hybridoma Sequencing with Reptor
We have sequenced over
Hybridomas and counting
Hybridoma Sequencing Service
Next-generation sequencing (NGS) platforms provide high-throughput, comprehensive, and in-depth coverage of antibody sequences in your hybridomas.
Benefits of hybridoma sequencing by NGS:
Deep sequencing each hybridoma reveals contaminating, minor clones that confound other sequencing methods.
Protect your IP
Sequencing all hybridomas from your campaign reveals related sequences that can contribute to a stronger patent application.
Never lose a hybridoma
Knowing the sequence of your valuable hybridomas gives you peace of mind in the event of hybridoma loss.
Low sample requirements
The sensitivity of next-generation sequencing enables our process to start from as few as 10,000 cells.
Our Aim: Give you access to every relevant antibody
Amplification and Sequencing
- Full-length variable region sequencing
- Mouse & Rat (IgG/IgM, IgK/IgL)
- Human (all isotypes)
- Rabbit (IgG, IgK)
- Transgenics (varies)
Analysis using proprietary software
- Error Correction and Repertoire analysis
- Full sequencing data access
Hybridoma Sequencing Process
- RNA Extraction starting from vials or plate
- Barcoding and Amplification
- Sequencing and Data Filtering
- Error Correction and Repertoire Analysis using our proprietary software
- >10k cells per hybridoma
Sample submission formats: Cryovials and plates
- Read more on how to properly ship your hybridomas
Turnaround time: 3 weeks
Work performed 100% in the USA!
Dominant and Minor Sequences for each hybridoma
Automated CDR detection on each sequence
Clonality assessment for each hybridoma
Isotype readout per hybridoma
Learn more on how you can save with our cost effective workflow!
Resources and Publications
Did you know many hybridomas are not monoclonal? We can determine clonality of your hybridoma with our process. Read more about a recent assessment of 185 hybridomas in When monoclonal antibodies are not monospecific: Hybridomas frequently express additional functional variable regions.