Careers

Responsibilities:

• Operate and maintain nano-flow liquid chromatography and high-resolution mass spectrometry (nano-LC-HRMS) instrumentation.
• Develop and validate of tandem MS-based analytical methods and standard operating procedures (SOPs) for discovery and targeted proteomics of complex antibody samples.
• Develop SOPs for LC-MS maintenance, operation and troubleshooting in a production setting.
• Collaborate with bioinformaticians and biologists to achieve technology milestones.
• Stay up to date on relevant literature in protein mass spectrometry, antibody discovery, and related areas.
• Communicate methods and results in presentations, publications, and posters.
• Contribute to our intellectual property portfolio.

Required Skills:

• PhD in chemistry, biochemistry, or similar with 3+ years of post-graduate work in academia or industry.
• Experience designing and implementing in-solution and in-gel digestion approaches to enable workflows for discovery proteomics.
• Strong analytical and practical skills in protein sample preparation and nano-LC-HRMS for proteomic analysis.
• Experience with Orbitrap type instrumentation and in-depth knowledge of instrument calibration, operation, troubleshooting, and maintenance (i.e. Q-Exactive, Orbitrap Fusion Lumos, Orbitrap Eclipse).
• Experience with various standard in-solution and in-gel proteomic sample prep protocols for bottom-up proteomic workflows.
• Ability to pick up new technologies quickly and troubleshoot independently.
• Proven track record of independent critical thinking, scientific achievement, and the ability to interact creatively and collaboratively with relevant departments.

Desired Skills:

• Experience with handling human as well as animal biomatrices (plasma/serum).
• Excellent analytical chemistry skills with a working knowledge and hands-on experience developing bottom-up and top/middle-down proteomic workflows using nano-flow LC-MS systems.
• Experience with labeled (TMT or iTRAQ) and label-free quantitative proteomic approaches (i.e. data-independent acquisition).
• Experience with data analysis software platforms, as well as third-party software platforms such as Max Quant, Trans-Proteomic Pipeline, Skyline, and Scaffold.
• Expertise with FPLC protein purification techniques such as IMAC, SEC, IEX, HIC.
• Expertise in preparing samples and performing liquid chromatography fractionation.
• Experience with high-throughput quantitative proteomic approaches; including sample multiplexing and modern liquid chromatography fractionation techniques.

Benefits:

• Competitive health and dental plans
• Employer retirement plan
• Flexible vacation
• On-site gym access

Responsibilities:

• Conduct lab work under the general direction of your supervisor.
• Recognize potential problems in order to understand and troubleshoot assays.
• Communicate clearly through an electronic lab notebook the protocols used and experimental results.
• Maintain an organized lab and work space.
• Collaborate with assay development scientists, bioinformatics scientists, and vendors to optimize and automate workflows.

Required Qualifications:

• B.S. in biochemistry, biology, genomics or similar field.
• 0-2 years post-undergraduate experience in life sciences.
• Experience with molecular biology techniques including RNA extraction, cDNA synthesis, PCR, and gel electrophoresis.
• Flexibility and curiosity to learn new techniques and workflows.

Preferred Qualification:

• Experience with preparing libraries for the Illumina MiSeq system.
• Experience in protein expression.
• Experience in protein chemistry and purification.
• Experience with Good Laboratory Practices.

Benefits:

• Competitive health and dental plans
• Employer retirement plan
• Flexible vacation
• On-site gym access