Rabbit Antibody Discovery with Alicanto

Rabbit Monoclonal Antibody Discovery from Serum

Abterra Bio offers custom monoclonal antibody discovery using Alicanto^®. You measure the success of your immunization using the serum antibody titer, so why focus on any rabbit antibodies not present in serum?

Convert rabbit pAbs to mAbs

Rabbit antibodies exhibit high affinity and specificity, and with 92 million years of evolutionary distance from human, can be raised against challenging targets.

Rabbit polyclonal antibodies are widely used in diagnostics and as reagents. Polyclonal antibodies are less expensive and faster to generate than monoclonals, however, polyclonals by their very nature have finite supply and are impossible to characterize fully.

We developed Alicanto, a monoclonal antibody discovery platform, to deliver monoclonal antibodies picked directly from a polyclonal antibody. Unlike B cell screening approaches, Alicanto directly analyzes antibody proteins in serum.

Alicanto delivers diverse monoclonal antibodies that replicate the behavior of the starting polyclonal antibody. Monoclonal antibodies can be fully characterized, IP protected, and produced recombinantly for an endless supply of material.

Alicanto rabbit antibody discovery overview

Verifiable Sequence Diversity

The natural immune response to vaccination is not uniform. Clonal families diversify and proliferate, and can account for an outsized proportion of the B cells and B cell transcripts. A sequence-blind screening approach such as phage display, B cell screening, or hybridomas will oversample the abundant, expanded clonal families and miss rare clones.

Sequence diversity is critical for identifying antibodies with diverse functions that bind diverse epitopes on the target antigen.

Alicanto performs sequencing as a standard intermediate step prior to selection. By being sequence-aware, Alicanto ensures the immune response is deeply analyzed, including rare clones that may exhibit a rare function.

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Deliverables

Natively Paired Heavy and Light Chain Sequences – Full sequence information for all hits identified by Alicanto®.

Interactive Report – An interactive report of the entire dataset to investigate individual antibodies, clonal families, and repertoire-wide features.

Transparency – Full access to the sequences and supporting data.

Validated Antibodies – Transient production of candidates and screening available.

Frequently

Asked

Questions

Why rabbit monoclonal antibody discovery?

Rabbits routinely produce antibodies with 10-100 times higher affinity than mouse antibodies, making them ideal for sensitive diagnostics and assays. Recombinant rabbit monoclonal antibodies provide consistency across batches and the security of knowing the antibody sequence. We immunize 2 animals per project and choose the rabbit with the best immune response for monoclonal discovery.

What are the material requirements?

We require 2.5 mg of antigen for immunization, affinity purification, and ELISA. The affinity purification can be customized to target specific variants, sub-domains, or cross-reactive species which would require additional antigen.

Can I send you samples from my existing immunization?

In some cases, we may be able to work with an immunization in progress, or a completed immunization. We can even discover antibodies without sacrificing the animal.