Antibody Protein Sequencing with Valens
De Novo Antibody Sequencing
Lost your hybridoma? For over a decade, our Valens™ platform has performed antibody protein sequencing to determine the primary amino acid sequence of monoclonal antibodies directly from the protein. Using mass spectrometry together with our patented spectral networking approach, Valens™ recovers full-length amino acid for heavy and light chains.
Isoleucine & Leucine Determination
Valens™ now supports CLIP™, a Bayesian approach to isoleucine and leucine determination.
Isoleucine and leucine are isomers, having identical mass but different structures. CLIP™ uses side-chain fragmentation of isoleucine and leucine to determine the correct amino acid during de novo antibody sequencing.
** CLIP is included for FREE with every project **
Antibody Sequencing & Expression
Material Requirements – 50ug of purified monoclonal antibody
Turnaround time – 3 weeks to sequence (expedited available)
Work performed 100% in the USA
We now offer our unique service in partnership with Antibody Design Laboratories. Transient production in a variety of formats and volumes available.
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Resources & Publications
Valens builds on over a decade of algorithm development including ideas invented at the University of California – San Diego Center for Computational Mass Spectrometry. For background on our methods check out these publications:
- Guthals, et al. (2012). Shotgun protein sequencing with meta-contig assembly. Mol Cell Proteomics, 11, 10:1084-96.
- Castellana, et al. (2011). Resurrection of a clinical antibody: template proteogenomic de novo proteomic sequencing and reverse engineering of an anti-lymphotoxin-α antibody. Proteomics, 11, 3:395-405.
- Castellana, et al. (2010). Template proteogenomics: sequencing whole proteins using an imperfect database. Mol Cell Proteomics, 9, 6:1260-70.
We recently summarized the approaches to de novo antibody sequencing in a blog post.