Antibody Protein Sequencing with Valens
De Novo Antibody Sequencing
Lost your hybridoma? Our Valens™ platform performs antibody protein sequencing to determine the primary amino acid sequence of your monoclonal antibody directly from the protein. Using mass spectrometry together with our patented spectral networking approach, Valens™ recovers full-length amino acid for heavy and light chains.
Antibody Sequencing & Expression
We now offer our unique service in partnership with LakePharma Inc. We will deliver your antibody sequence with validated expression and binding, guaranteed.
Material Requirements – 200ug of purified monoclonal antibody
Turnaround time – 5 weeks to sequence
Work performed 100% in the USA
Isoleucine & Leucine Determination
Valens™ now supports CLIP™, a Bayesian approach to isoleucine and leucine determination.
Isoleucine and leucine are isomers, having identical mass but different structures. CLIP™ uses side-chain fragmentation of isoleucine and leucine to determine the correct amino acid during de novo antibody sequencing.
Resources & Publications
Valens builds on over a decade of algorithm development including ideas invented at the University of California – San Diego Center for Computational Mass Spectrometry. For background on our methods check out these publications:
- Guthals, et al. (2012). Shotgun protein sequencing with meta-contig assembly. Mol Cell Proteomics, 11, 10:1084-96.
- Castellana, et al. (2011). Resurrection of a clinical antibody: template proteogenomic de novo proteomic sequencing and reverse engineering of an anti-lymphotoxin-α antibody. Proteomics, 11, 3:395-405.
- Castellana, et al. (2010). Template proteogenomics: sequencing whole proteins using an imperfect database. Mol Cell Proteomics, 9, 6:1260-70.
We recently summarized the approaches to de novo antibody sequencing in a blog post.