Antibody Repertoire Sequencing and Analysis
The natural B-cell receptor repertoire is a wealth of information on the immune state of an individual. Abterra Bio now offers Reptor™, an end-to-end solution for B-cell receptor sequencing, repertoire construction, and analysis.
We can work with any species including transgenics!
Reptor™ begins by extracting RNA from tissues such as bone marrow, lymph nodes, or spleen, or directly from cells. Our software automatically constructs a B-cell receptor repertoire by stitching read pairs, correcting errors, and filtering by quality.
Automated Repertoire Analysis
Each antibody can be analyzed individually, or the repertoire can be visualized using compiled statistics and presentation-ready plots.
Reptor™ service provides:
- Interactive repertoire results available through our proprietary software.
- Complete antibody repertoire downloadable from online.
Read more below on how Alicanto® can further improve your antibody discovery
A Tale of Two Repertoires
The B-cell receptor repertoire is a snapshot of transcriptionally active B cells and plasma cells. The repertoire of antibodies present in serum is a distinct collection of molecules that tells a different story.
We developed Alicanto® to integrate next-generation sequencing of B-cell receptors with mass spectrometry-based analysis of circulating antibodies. We apply this platform to discover high-affinity antibodies.
Resources and Publications
Check out our blog posts related to antibody repertoire sequencing and analysis: