Bulk B-Cell Sequencing with Reptor
B-Cell Receptor Repertoire Sequencing
Bulk B cell sequencing uses next-generation sequencing to profile the antibody repertoire. Dive deeper into profiling your sorted B cell populations, and tissues by sequencing isotype-specific antibodies.
Check out our latest blog post on immune repertoire sequencing in human!
Features
Species and Samples
- Extensible to any species including transgenics
- Samples can start from cells or tissue including bone marrow, spleen, lymph node, and blood
Amplification and Sequencing
- Full-length variable region sequencing
- Isotypes:
- Mouse & Rat (IgG/IgM, IgK/IgL)
- Human (all isotypes)
- Rabbit (IgG, IgK)
- Transgenics (varies)
Analysis using proprietary software
- Error Correction and Repertoire analysis
- Full sequence data access
Bulk B Cell Sequencing Workflow
Protocol
- RNA Extraction and cDNA Synthesis
- Either Amplicon Generation or Ig Amplification
- Sequencing and Data Filtering
- Error Correction and Repertoire Analysis using our proprietary software
Material Requirements:
- 100 – 5M Cells
- Tissue pieces should be no larger than 1 cm in any dimension
Sample submission formats: Cryovials and plates
- Read more on how to properly ship your cells and tissues
Turnaround time: 3 weeks
Key Benefits
Deep sequence polyclonal antibody samples
Discover novel variants of hybridoma hits
Enabling antibody sequencing beyond mouse and human
Complete antibody repertoire with germline gene and CDR annotations
Work performed 100% in the USA
Get In Touch
Tell Us About Your Project.
Need more information? We’re here to answer any questions you have.
Resources and Publications
Our blog posts related to antibody repertoire sequencing and analysis:
From RNA to sequencing: QC matters
From reads to antibodies: Constructing an accurate immune repertoire
Single-molecule Ig repertoire sequencing from Lama glama