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Antibody Reference Guide

Antibody Reference Guide

Antibody Reference Guide We developed a one-sheet antibody reference that is handy for antibody-related work. We refer to it often to remind ourselves of the major antibody isotypes, antibody fragments, and purification strategies, as well as antibody development...
Next Generation Sequencing-Enhanced Antibody Discovery

Next Generation Sequencing-Enhanced Antibody Discovery

Using Reptor for NGS-enhanced antibody discovery Traditional antibody discovery methods, such as hybridoma and phage display, as well as newer single cell screening methods, capture only a small portion of the adaptive immune response, and as a result isolate a small...
BCR Repertoire Sequencing: Error Correction

BCR Repertoire Sequencing: Error Correction

BCR Repertoire Sequencing: Error Correction Sequencing errors: where do they come from? Immune repertoire sequencing (Rep-seq) allows us to deconvolute the population of antibody sequences that comprise an individual’s B-cell response. While antibody repertoire...
VHH Discovery By Serum Proteomic Analysis

VHH Discovery By Serum Proteomic Analysis

Llama VHH discovery from serum Camelids, such as llamas and alpacas, naturally produce both conventional antibodies and heavy-chain only antibodies (HCAbs)(1). The antigen-binding domain of the HCAb, called the VHH or nanobody, is comprised of a single polypeptide...
I/L Determination in De Novo Antibody Sequencing

I/L Determination in De Novo Antibody Sequencing

I/L Determination in De Novo Antibody Sequencing Isoleucine and leucine determination De novo antibody sequencing by tandem mass spectrometry(MS/MS) uses the differences in mass of different amino acids to accurately determine the correct sequence. Distinguishing...

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