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Reptor™- BCR Repertoire Sequencing

Bulk B cell sequencing uses next-generation sequencing to profile the antibody repertoire. Dive deeper into profiling your sorted B cell populations, and tissues by sequencing isotype-specific antibodies.

Check out our latest blog post on immune repertoire sequencing in human!

Get started on your project.

Our partners’ success means everything to us!

 

I have had great experiences using Abterra Bio’s Reptor platform. The data generated was highly valuable, and the team provided quick and open communication throughout the whole process.

Sandeep Kumar, PhD

Associate Director - Antibody Discovery & Engineering, Scholar Rock

Features

Reptor acceptable species include mouse, rat, rabbit, human, transgenics

Species and Samples

  • Species: extensible to any species including transgenics
  • Samples: can start from cells or tissue including bone marrow, spleen, lymph node, and blood
  • Contact us if you are interested!
Next-generation sequencing for hybridomas

Amplification and Sequencing

  • Full-length variable region sequencing
  • Isotypes:
    • Mouse & Rat (IgG/IgM, IgK/IgL)
    • Human (all isotypes)
    • Rabbit (IgG, IgK)
    • Transgenics (varies)
Proprietary Reptor software

Analysis using proprietary software

BCR Repertoire Sequencing Process

 

Input

RNA extraction starting from vials or plate

Amplification

Barcoding and amplification

Sequence

Sequencing and filtering

Analysis

Error correction and repertoire analysis using our proprietary software

Deliverables

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Files of every sequence discovered

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Interactive html report with repertoire analysis

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Automated diversity analysis: gene usage, CDR3 lengths, mutation counts and more!

3 week turnaround time!

 * Work performed 100% in the USA!

Other Reptor™ Services

The natural B-cell receptor repertoire is a wealth of information on the immune state of an individual. Click below to learn more about how our services can help you achieve your project goals!

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