Reptor™- BCR Repertoire Sequencing
Bulk B cell sequencing uses next-generation sequencing to profile the antibody repertoire. Dive deeper into profiling your sorted B cell populations, and tissues by sequencing isotype-specific antibodies.
Check out our latest blog post on immune repertoire sequencing in human!
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Our partners’ success means everything to us!
I have had great experiences using Abterra Bio’s Reptor platform. The data generated was highly valuable, and the team provided quick and open communication throughout the whole process.
Features
Species and Samples
- Species: extensible to any species including transgenics
- Samples: can start from cells or tissue including bone marrow, spleen, lymph node, and blood
- Contact us if you are interested!
Amplification and Sequencing
- Full-length variable region sequencing
- Isotypes:
- Mouse & Rat (IgG/IgM, IgK/IgL)
- Human (all isotypes)
- Rabbit (IgG, IgK)
- Transgenics (varies)
Analysis using proprietary software
- Error Correction and Repertoire analysis
- Full sequencing data access
BCR Repertoire Sequencing Process
Input
RNA extraction starting from vials or plate
Amplification
Barcoding and amplification
Sequence
Sequencing and filtering
Analysis
Error correction and repertoire analysis using our proprietary software
Deliverables
Files of every sequence discovered
Interactive html report with repertoire analysis
Automated diversity analysis: gene usage, CDR3 lengths, mutation counts and more!
3 week turnaround time!
* Work performed 100% in the USA!
Other Reptor™ Services
The natural B-cell receptor repertoire is a wealth of information on the immune state of an individual. Click below to learn more about how our services can help you achieve your project goals!
Get In Touch
Tell Us About Your Project.
Need more information? We’re here to answer any questions you have.
Our Location
11588 Sorrento Valley Rd
Ste 16
San Diego, CA 92121
Our blog posts related to antibody repertoire sequencing and analysis:
Immune repertoire sequencing in human
From RNA to sequencing: QC matters
From reads to antibodies: Constructing an accurate immune repertoire