MotivationSince the technology's invention in 1975, hybridomas have been a favored method of monoclonal antibody production. By fusing immortal myeloma cells with antibody producing B cells in culture, hybridomas can deliver a nearly infinite supply of antibodies....

MotivationHuman antibody sequencing is a powerful tool for understanding the humoral immune response. Until recently, B cell-focused approaches have been the only available tool for understanding the diversity and plasticity of the antibody response to challenge (see...

Fasta2airr is a free web app for converting antibody sequences into annotated AIRR-Seq files. Converting sequences into this open format is an essential step in antibody repertoire analysis. Among other antibody meta data the AIRR-Seq file contains: Germline gene...

Serum contains circulating antibodies with unique functional properties. This fundamental fact is why we choose to mine the serum over cells - despite being more challenging. Here we compare the two approaches for polyclonal antibody sequencing available at Abterra...

Protein sequencing has been possible for 75 years with Edman degradation, but over the past decade, mass spectrometry (MS)-based sequencing has become the dominant approach for antibodies. The general strategy is to digest antibody proteins into short peptides using...