Scientific News
De Novo Antibody Sequencing NISTmAb with Post-Translational Modification Detection
Protein sequencing has been possible for 75 years with Edman degradation, but over the past decade, mass spectrometry (MS)-based sequencing has become the dominant approach for antibodies. The general strategy is to digest antibody proteins into short peptides using...
Monoclonal versus Polyclonal Antibody Sequencing
Antibody reagents used in biomedical research and diagnostics assays fall into two large categories: monoclonals and polyclonals. For high-quality reagents, an antibody needs to demonstrate specific reactivity in its intended assay, and maintain consistency across...
Polyclonal Antibody Sequencing Methods
Polyclonal Antibody Sequencing Methods Polyclonal antibody sequencing is a powerful tool for understanding the humoral immune response.1 Two primary approaches exist: Proteogenomic Polyclonal Antibody Sequencing and De Novo Polyclonal Antibody Sequencing. This guide...
Griffin: Accessing functional antibodies from serum without B cells
Griffin is a platform for sequencing polyclonal antibodies using mass spectrometry. Since 2009, Abterra Biosciencs founders and employees have been developing novel methods for sequencing antibody proteins directly. Our monoclonal antibody sequencing service,...
Antibody Clonality Assessment
Antibody Clonality Background The serological compartment is simple to access and contains a multitude of proteins, a large fraction of which are circulating antibodies. At Abterra Bio, we developed Alicanto which utilizes B cells from a variety of sources, including...
Unlocking the Secrets of SARS-CoV-2 Immunity: A Deep Dive into Serum Antibodies and B Cell Repertoires
Project Summary We're excited to share our latest findings on the complex interplay between serum antibodies and B cell repertoires in individuals with SARS-CoV-2 immunity. Our study offers new insights into the development of high-affinity antibodies against emerging...
VHH Discovery with Alicanto versus Phage Display
Project Summary Camelids such as llamas and alpacas produce conventional antibodies as well as heavy-chain only antibodies (HCAbs) from which the small, stable, antigen-binding domain (VHH) can be extracted. Target-specific VHHs can be identified from immunized...
Limit of Detection – Serum Antibody Analysis
In this blog post, we evaluate the limit of detection for serum antibody analysis achieved by Alicanto using spike ins.
VHH Antibody Repertoire to Phage Library Comparison
We used Reptor to compare the VHH antibody repertoire from an immunized llama to a phage library constructed from the same source.
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