888-416-9305 info@abterrabio.com

Reptor™- Hybridoma Sequencing 

We give you access to every relevant antibody for your project!

  • 3 week turnaround time
  • Starting at $110/hybridoma
  • All work performed in the USA!

 

We have sequenced

hybridomas and counting

 

Speak to a scientist

 

Our partners’ success means everything to us!

 

I have had great experiences using Abterra Bio’s Reptor platform. The data generated was highly valuable, and the team provided quick and open communication throughout the whole process.

Sandeep Kumar, PhD

Associate Director - Antibody Discovery & Engineering, Scholar Rock

Features

Reptor acceptable species include mouse, rat, rabbit, human, transgenics

Species and Samples

  • Species: Mouse, Rat, Rabbit, Human, and Transgenics
  • Contact us if you are interested in sequencing hybridomas from a different species!
  • Samples: cells
Next-generation sequencing for hybridomas

Amplification and Sequencing

  • Full-length variable region sequencing
  • Isotypes:
    • Mouse & Rat (IgG/IgM, IgK/IgL)
    • Human (all isotypes)
    • Rabbit (IgG, IgK)
    • Transgenics (varies)
Proprietary Reptor software

Analysis using proprietary software

Hybridoma Sequencing Process

 

Input

RNA extraction starting from vials or plate

Amplification

Barcoding and amplification

Sequence

Sequencing and filtering

Analysis

Error correction and repertoire analysis using our proprietary software

* Read about how next-generation sequencing is better than Sanger sequencing for determining monoclonal antibodies in your hybridomas!

Deliverables

i

Dominant and Minor Sequences for each hybridoma

i

Automated CDR detection on each sequence

i

Clonality assessment for each hybridoma

i

Isotype readout per hybridoma

3 week turnaround time!

 * Work performed 100% in the USA!

Other Reptor™ Services

The natural B-cell receptor repertoire is a wealth of information on the immune state of an individual. Click below to learn more about how our services can help you achieve your project goals!

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