Reptor™- Hybridoma Sequencing

We give you access to every relevant antibody for your project!

3 week turnaround time
Starting at ~$140/hybridoma
All work performed in the USA!

We have sequenced

hybridomas and counting

Get started on your project.

Our partners’ success means everything to us!

I have had great experiences using Abterra Bio’s Reptor platform. The data generated was highly valuable, and the team provided quick and open communication throughout the whole process.

Sandeep Kumar, PhD

Associate Director - Antibody Discovery & Engineering, Scholar Rock

Features

Reptor acceptable species include mouse, rat, rabbit, human, transgenics

Species and Samples

Species: Mouse, Rat, Rabbit, Human, and Transgenics
Contact us if you are interested in sequencing hybridomas from a different species!
Samples: cells

Next-generation sequencing for hybridomas

Amplification and Sequencing

Full-length variable region sequencing
Isotypes:
- Mouse & Rat (IgG/IgM, IgK/IgL)
- Human (all isotypes)
- Rabbit (IgG, IgK)
- Transgenics (varies)

Analysis using proprietary software

Error Correction and Repertoire analysis
Full sequencing data access

Hybridoma Sequencing Process

Input

RNA extraction starting from vials or plate

Amplification

Barcoding and amplification

Sequence

Sequencing and filtering

Analysis

Error correction and repertoire analysis using our proprietary software

* Read about how next-generation sequencing is better than Sanger sequencing for determining monoclonal antibodies in your hybridomas!

Deliverables

i

Dominant and Minor Sequences for each hybridoma

i

Automated CDR detection on each sequence

i

Clonality assessment for each hybridoma

i

Isotype readout per hybridoma

Get In Touch

Tell Us About Your Project.

Need more information? We’re here to answer any questions you have.

SPEAK TO A SCIENTIST

Other Reptor™ Services

The natural B-cell receptor repertoire is a wealth of information on the immune state of an individual. Click below to learn more about how our services can help you achieve your project goals!