Hybridoma Sequencing Service

POWERED BY REPTOR

What sets our hybridoma sequencing service apart?

EXPERIENCE: We’ve sequenced thousands of hybridomas and built optimize workflows to handle every tricky case.
SENSITIVITY: We deeply sequence each clone, suppressing irrelevant myeloma transcripts, to capture rare sequences.
THROUGHPUT: Our plate-based workflow can handle hundreds of clones per project.
FLEXIBILITY: Our expert scientists can adapt our workflow to your needs – including custom species, strains, and all isotypes.

Our hybridoma sequencing service uses high-throughput NGS technology.

Key Features:

Full variable region sequencing, with isotype call.
Available for mice, rats, rabbits, humans, hamsters, and transgenics.
Suppression of myeloma cell antibody transcripts.
All work performed in the USA!

We have sequenced over

hybridomas and counting

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Hybridoma Sequencing Service Process

Input

RNA extraction starting from vials or plate

Amplification

Barcoding and amplification

Next-generation sequencing for hybridomas.

Sequence

Sequencing and filtering

Analysis

Error correction and repertoire analysis using our proprietary software

Deliverables

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Dominant and Minor Sequences for each hybridoma

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Automated CDR detection on each sequence

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Clonality assessment for each hybridoma

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Isotype readout per hybridoma

Hybridoma sequencing service result table

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