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 Antibody Sequencing Service

Do you have antibody you like but need a sequence? The Abterra Bio scientists have spent decades honing methods for our antibody sequencing service.  Whether you have hybridomas, B cells, DNA, RNA, or protein – we can help!

REPTOR

For next-generation sequencing of cells, DNA, or RNA, Reptor is a flexible platform to deliver high quality antibody sequences.  Click below to read more about specific antibody sequencing applications powered by Reptor.

VALENS

For antibody sequencing directly from the protein, Valens is a monoclonal antibody sequencing service that uses mass spectrometry to deliver high-confidence antibody sequences and expressed antibodies.

For our polyclonal antibody sequencing service, see our service Griffin.

What are the different types of antibody sequencing services?

Next-generation sequencing methods

If you have access to nucleic acids, then next-generation sequencing-based services, like Reptor, are ideal.  With NGS, Reptor delivers accurate sequences for projects both small and large.  While there are myriad variations, these methods typically follow a standard process:

  1. amplicon generation
  2. sequencing
  3. bioinformatics analysis
  4. expression
Polyclonal antibody sequencing stage 1 sequences CDR3s and clusters similar antibodies to assess clonality.

Protein sequencing methods

Mass spectrometry is the method of choice for proteomics, and utilized by our protein antibody sequencing services, Valens and Griff.

For monoclonal antibody sequencing, Valens is the culmination of nearly 20 years of algorithm development along side mass spectrometry method optimization.

For polyclonal antibody sequencing, Griffin combines bottom-up and middle-down mass spectrometry methods to assemble antibody sequences from complex mixtures.

Many peptides are assembled to perform polyclonal antibody sequencing.  The image shows the map of peptides to a final sequence.

Have both cells and protein?

 

Alicanto is a sensitive, accurate technology that uses both B cells and serum for polyclonal antibody sequencing and antibody discovery.

If you have B cells, PBMCs, or access to the host animal, Alicanto is an ideal option for custom antibody development.

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