FREE ANALYSIS SOFTWARE!
AIRRSeq Viewer
The AIRRSeq Viewer is free software for visualizing antibody repertoires when spreadsheets just won’t cut it.
Built by antibody researchers with the core functionalities you need to progress your antibody discovery and analysis projects.
AIRRSeq Viewer Tutorials
1. Sorting, Filtering, and Exporting
Take a tour of the Repertoires pane to sort, filter, and export your sequences in FASTA format or tab-delimited AIRR-Seq format.
2. Finding Sequences Related to a Query
Use the Quick Filter to find sequences related to your query sequence or selected hit.
3. Generating Repertoire-Wide Statistics
Using a llama VHH repertoire as an example, learn how to use the Summary pane to generate repertoire-wide distributions of germline gene usage, CDR3 lengths, mutation counts, and more.
4. Identifying Clonal Lineages
Identify all members of a clone found across different stages of the hybridoma sequencing process. Visualize each lineage as a tree.
5. Clustering on CDR3 Similarity
The Clone Clusters tab is used to find similar CDR3s. Select the clustering parameters, cluster sequences, and explore connected components with related CDR3 sequences
6. Finding Related Clones to a Hit
In this video, we used clone clustering to identify relatives of single B cell screening hits in a rabbit bulk repertoire.
Demo Data
COMING SOON
Rabbit heavy chain repertoire from multiple sources including PBMCs, Bone Marrow, Spleen, and single B cell screening (~400 entries)
Frequently Asked Questions
What file formats are accepted by AIRRSeq Viewer?
The AIRRSeq Viewer requires a tab delimited, AIRR community file format (airr.tsv) or gzipped (airr.tsv.gz). If you need help getting your repertoire into this format, you can use the fasta2airr web app or contact us for support.
You can check if your AIRR formated file is valid in the AIRRSeq Viewer using File -> Check AIRR-seq…
Do the germline gene references need to be from a specific database?
No. You can use your own naming convention or even custom germline genes for the repertoire.
What columns are needed in the AIRR file format?
The AIRR Community has defined AIRR Rearrangement Schema for describing B and T cell receptor sequences. This format describes the columns. The column order does not matter for the AIRRSeq Viewer, but the column names must match the schema.
How do I get extra help with my analysis?
Contact our scientific team for specialized support for your projects. Email us at support@abterrabio.com or through our Contact Us page.
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