Alicanto® is Abterra Biosciences' proprietary antibody discovery platform that mines the entire immune response to identify diverse, functional antibodies. For an overview of the proteogenomic process, read more about Alicanto®. Alicanto benefits include: Full...

We developed a one-sheet antibody reference that is handy for antibody-related work. We refer to it often to remind ourselves of the major antibody isotypes, antibody fragments, and purification strategies, as well as antibody development processes. Below we outline...

Using Reptor for NGS-enhanced antibody discoveryTraditional antibody discovery methods, such as hybridoma and phage display, as well as newer single cell screening methods, capture only a small portion of the adaptive immune response, and as a result isolate a small...

Sequencing errors: where do they come from?Immune repertoire sequencing (Rep-seq) allows us to deconvolute the population of antibody sequences that comprise an individual’s B-cell response. While antibody repertoire diversity is known to be vast, errors can cause a...

Llama VHH discovery from serumCamelids, such as llamas and alpacas, naturally produce both conventional antibodies and heavy-chain only antibodies (HCAbs)(1). The antigen-binding domain of the HCAb, called the VHH or nanobody, is comprised of a single polypeptide...

Isoleucine and leucine determinationDe novo antibody sequencing by tandem mass spectrometry(MS/MS) uses the differences in mass of different amino acids to accurately determine the correct sequence. Distinguishing between isoleucine (Ile) and leucine (Leu) residues is...

In recent years, camelids have become more than hearty travel companions due to their unconventional adaptive immune systems. Conventional IgG antibodies are tetrameric molecules comprised of two heavy chains and two light chains with molecular weights of roughly ~150...

What is an antibody repertoire?The immune repertoire is the collection of unique immunoglobulin and T-cell receptor sequences present in an individual at a particular time. At Digital Proteomics we focus primarily on the immunoglobulin, or B-cell receptor repertoires...

Sequencing the immunoglobulin (Ig) repertoire provides us with important information on the adaptive immune response, and can help with the development of diagnostic and therapeutic applications. A single human can have an estimated 10¹¹ B cells with millions of...