Sequencing errors: where do they come from?Immune repertoire sequencing (Rep-seq) allows us to deconvolute the population of antibody sequences that comprise an individual’s B-cell response. While antibody repertoire diversity is known to be vast, errors can cause a...

Llama VHH discovery from serumCamelids, such as llamas and alpacas, naturally produce both conventional antibodies and heavy-chain only antibodies (HCAbs)(1). The antigen-binding domain of the HCAb, called the VHH or nanobody, is comprised of a single polypeptide...

Isoleucine and leucine determinationDe novo antibody sequencing by tandem mass spectrometry(MS/MS) uses the differences in mass of different amino acids to accurately determine the correct sequence. Distinguishing between isoleucine (Ile) and leucine (Leu) residues is...

In recent years, camelids have become more than hearty travel companions due to their unconventional adaptive immune systems. Conventional IgG antibodies are tetrameric molecules comprised of two heavy chains and two light chains with molecular weights of roughly ~150...

What is an antibody repertoire?The immune repertoire is the collection of unique immunoglobulin and T-cell receptor sequences present in an individual at a particular time. At Digital Proteomics we focus primarily on the immunoglobulin, or B-cell receptor repertoires...

Sequencing the immunoglobulin (Ig) repertoire provides us with important information on the adaptive immune response, and can help with the development of diagnostic and therapeutic applications. A single human can have an estimated 10¹¹ B cells with millions of...

If you search the internet for ‘antibody sequencing’, you will be rewarded with a long list of resources and services. The terms used to describe antibody sequencing are overloaded and highly similar. We felt a single resource that explained the why and how of the de...